DN2M » Technology » Methodological plateform » Molecular and Cellular imaging

Molecular and Cellular imaging

The cell imaging centre BICEL-IFR114, IBISA certified, offers different activities as trainings, support, monitoring and consulting to a wide scientific community present within the site but also to national and international entities academic organizations or private companies. Studies using imaging equipment such as confocal microscope, conventional microscope or times series microscope are designed to localize, by immunofluorescence techniques, fusion proteins or histological stainings, molecules of interest in tissues and cells, fixed or alive. These systems also allow the visualization and quantification of many dynamic processes in biology such as cell survival, cell migration, changes in membrane potential or intracellular calcium concentration. Experiments of FRET, FRAP or photo-manipulation (photo-activation, photo-conversion) are also achievable through advanced microscopy techniques dynamics. The platform also offers studies in molecular biology with the technique of laser microdissection coupled with quantitative PCR in order to quantify the relative expression of genes in tissues or cells.

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Confocal microscopy analysis of Tau localization in cultured neurons.

References

Büttner S, Delay C, Franssens V, Bammens T, Ruli D, Zaunschirm S, de Oliveira RM, Outeiro TF, Madeo F, Buée L, Galas MC, Winderickx J, “Synphilin-1 enhances α-synuclein aggregation in yeast and contributes to cellular stress and cell death in a Sir2-dependent manner”, (2010) PLoS One. 27, e13700

Sultan A, Nesslany F, Violet M, Bégard S, Loyens A, Talahari S, Mansuroglu Z, Marzin D, Sergeant N, Humez S, Colin M, Bonnefoy E, Buée L, Galas MC, “Nuclear tau, a key player in neuronal DNA protection”, (2011) J Biol Chem. 3, 4566-4575

Vandenhaute E, Dehouck L, Boucau MC, Sevin E, Uzbekov R, Tardivel M, Gosselet F, Fenart L, Cecchelli R, Dehouck MP, “Modelling the neurovascular unit and the blood-brain barrier with the unique function of pericytes”, (2011) Curr Neurovasc Res. 8, 258-269